Cytotoxic screening of endophytic fungi associated with Catharanthus roseus

Abstract. Lay CS, Agustina I, Astuti P, Hertiani T. 2023. Cytotoxic screening of endophytic fungi associated with Catharanthus roseus. Biodiversitas 24: 2716-2722. Despite the advancements in cancer therapy, there is still a need for new anticancer agents. Due to tremendous range chemical compounds that create, they have become crucial component current drug development process. The aim this study was evaluate cytotoxic potential roseus (L.) G.Don collected from Yogyakarta, Indonesia. All fungal EtOAc extracts were tested against three human cell lines, HeLa, T47D, MCF-7, and Vero lines. isolate identified using molecular techniques. results showed total seven obtained different parts C. Only one fungus (SCR 3) best activitiy towards all lines used, especially MCF-7 line (IC50 130.90 ?g/mL, Selectivity Index=11.42). findings LC-HRMS also revealed several exciting such as genistein D-(-)-Mannitol. extract SCR 3 (Aspergillus sp.) induced apoptosis but did not affect cycle. This suggests may be source secondary metabolites can function